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Plasmid Cap Bestarose HP - Affinity Resins

Plasmid Cap Bestarose HP - Affinity Resins

Catalog Number:
CM01502185BES
Mfr. No.:
AA0201-AA116314
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      • Overview

        • Plasmid Cap Bestarose HP is a thiophilic affinity resin made by fixing sulfur-containing compound 2-mercapyridine on high resolution crosslinked agratose. Its optimized ligand density has appropriate affinity with superhelix DNA, and fine particle microspheres can improve the binding capacity of superhelix DNA with larger molecular weight. The principle of thiophilic affinity is to use the interaction between electron donor and electron acceptor to separate and purify biomolecules. This force is strengthened in high salt environment and weakened in low salt environment.

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      • Properties
        • Categories
          Affinity Resins
          Other Properties
          Appearance: White slurry
          Matrix: Highly Cross-linked agarose, 6%
          Average particle size: ~34μm
          Functional group: 2-mercaptopyridine
          Ligand concentration: ~3.5mg 2-mercaptopyridine /mL resin
          Dynamic binding capacity: ＞2mg Supercoiled plasmid DNA /mL packed resin
          Chemical Stability: Stable in common aqueous buffers: 1M HAC+ ,30% isopropyl alcohol, 70% ethanol , 0.1M NaOH
          Max. pressure: 0.3MPa
          pH stability: 3~11 (Working), 2~13 (CIP)
          Storage++: 2~30°C, 20% ethanol or 2% benzyl alcohol
          Operating temperature: 15~30°C

          +1M 1M HAc only be used for cleaning.
          ++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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