Bull 4MID® Kit (proAKAP4 marker)

- Overview
  - The Bull 4MID® Kit (Ref. 4VDX-18K4) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of bull semen.
    
    This ELISA kit is the 1st functional test to evaluate sperm quality and fertility, as high levels of AKAP4 has been shown in high fertile bulls (Peddinti et al. 2008 : Singh et al. 2019 ; Ruelle et al. 2019 ; Ruelle et al. 2020 ; Bastan and Akcay, 2021 ; Marques de Almeida, 2022 ; Dordas-Perpinya et al. 2022).
    
    The dosage of the proAKAP4 biomarker with the Bull 4MID® Kit, will bring :
    
    • Objective information on semen quality in bulls (all breeds)
    • Qualification of each breeding male and their ejaculates overtimes
    • Selection of semen with long-lasting motility at post-thaw
    • Pertinent parameter to improve sperm cryopreservation
    • Selection of semen with less tail abnormalities
    • A functional indicator correlated to fertility
    
    The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al. 2019 ; Bastan and Akcay, 2021).
    High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in bull semen samples (Bastan and Akcay, 2021).
    
    Please contact us at for specific academic pricing.
    
    Background
    
    ProAKAP4 (A–kinase anchor protein 4) has been described over the last decade as a functional marker of spermatozoa. This key protein is largely conserved between species and its specific regulation during spermatogenesis seems essential to increase success rate in artificial insemination settings. Recent cross species analyses have identified the potential use of proAKAP4 as a pertinent biomarker of overall semen quality in clinical settings. In terms of structure, proAKAP4 is a precursor polypeptide that must be converted by motile and alive spermatozoa in mature AKAP4 that consequently will dock and coordinate the core transduction signals regulating sperm functionality, i.e motility, capacitating and fertility.
    
    1. Luconi M, Cantini G, Baldi E, et al. Role of A-kinase anchoring proteins (AKAPs) in reproduction. Front Biosci. 2011;16:1315–1330.
    2. Sergeant N, Briand-Amirat L, Bencharif D, et al. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy and Veterinary Science. 2019;11:01‒19.
    3. Delehedde M, Blommaert D, Jouy N, et al. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Rep Sci. 2018;194:224.
    4. Delehedde M, Demouveaux B, Remy G, Selleslagh M et al. ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. 2019;7(1):86.
    
    More Details
    
    4MID® ProAKAP4 ELISA Kits
- Properties
  - Details
    
    SPECIFICITY: Bull proAKAP4 (all breeds)
    ANALYTICAL RANGE: 9 to 150 ng
    SAMPLE TYPE: Fresh, chilled or frozen semen / Isolated spermatozoa
    
    * Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
- Applications
  - Application
    
    ELISA
    
    Application Description
    
    The Bull 4MID® Kit allows you to quantify proAKAP4 in samples of bull semen as a biomarker of sperm quality and fertility.
    This ELISA kit allow to assess from 1 to 88 samples simultaneously or individually (12 strips of 8 wells per microplate 96 wells / per kit)
    Working with ejaculate, chilled or frozen semen
- Reference
  - Dordas-Perpinyà M, Sergeant N, Ruelle I, Bruyas JF, Charreaux C, Michaud S, Carracedo S, Catalán J, Miró J, Delehedde M and Briand-Amirat L (2022) ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility: a retrospective study. Veterinary Sciences. Vol. 9 :224.
    Dordas-Perpinyà M, Sergeant N, Ruelle I, Bruyas JF, Charreaux C, Michaud S, Carracedo S, Catalán J, Miró J, Delehedde M and Briand-Amirat L (2022) ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility: a retrospective study. Veterinary Sciences. Vol. 9 :224.
    Marques de Almeida AB, Megumy Tsunokawa HidalgoM, Zito de Moraes FL, Corsi Trautwein LG, Schnitzer J, Dos Santos Silva LA, Rizzoto G, Pinheiro Ferreira JC and Mello Martins MI (2022) The proAKAP4 concentrations in Nellore bulls' sperm and its relations to FTAI conception rates results. Rev Bras Reprod Anim. Vol. 46(2)178-179.
    Kowalczyk A, Gałeska E and Bubel A (2022) The Concentration of ProAKAP4 and Other Indicators of Cryopotential of Spermatozoa Cryopreserved in Extender with Holothuroidea Extract Addition. Animals. Vol. 12:521. https:// doi.org/10.3390/ani12040521
    Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164
    Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19.
    Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
    Singh R, Junghare V, Hazra S, Singh U, Sengar GS, Raja TV, Kumar S, Tyagi S, Das A.K., Kumar A, Koringa P, Jakhesara S, Joshi CJ and Deb R (2019) Database on spermatozoa transcriptogram of catagorised frieswal crossbred (holstein friesian X sahiwal) bulls. Theriogenology. Vol. 129:130-145.
    Fu Q, Pan L, Huang D, Wang Z, Hou Z and Zhang M (2019) Proteomic profiles of buffalo spermatozoa and seminal plasma. Theriogenology. Vol. 134:74-82.
    Ruelle I, Charreaux F, Bencharif D, Thorin C, Michaud S, Schmitt E, Sergeant N, Briand-Amirad L and Delehedde M (2019) Assessment of the sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Holstein bulls. Animal Reproduction. In press.
    Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24.
    Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135.
    Jagan Mojanarao G and Atreja S.K (2011) Identification of capacitation associated tyrosine phosphoproteins in buffalo and cattle spermatozoa. Animal Reproduction Science. Vol. 123:40-47.
    Fiedler S, Bajpai M and Carr D (2008) Identification and characterization of RHOA-interacting proteins in bovine spermatozoa. Biology of Reproduction. Vol. 78:184-192.
    Peddinti D, Nanduri B, Kaya A, Feugang JM, Burgess SC and Memili E (2008) Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. BMC Systems Biology. Vol. (2)19:1-13.
    Moss S, Turner R, Burkert K, Butt H and Gerton G (1999) Conservation and function of a bovine sperm A-kinase anchor protein homologous to mouse AKAP82. Biology of Reproduction. Vol. 61:335-342.

Bull 4MID® Kit (proAKAP4 marker)

Bull 4MID® Kit (proAKAP4 marker)

Background

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