Phenyl Bestarose HP - Phenyl HIC Resins

Phenyl Bestarose HP - Phenyl HIC Resins

Catalog Number:
CM01502225BES
Mfr. No.:
AH201105-AH201116
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      • Overview
        • Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
          Phenyl Bestarose HP is based on highly cross-linked agarose, which retains excellent hydrophilicity and pore structure of natural polysaccharide compounds while having good compatibility with biological macromolecules. The average particle size of Phenyl Bestarose HP is 34μm, which enjoys higher resolution efficiency than Phenyl Bestarose FF. The surface contains Phenyl hydrophobic ligands, which are particularly suitable for the polishing and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Phenyl HIC Resins
          Other Properties
          Appearance: White slurry
          Matrix: Cross-linked agarose, 6%
          Particle size+: 24~44μm (the average particle size is 34μm)
          Functional group: Phenyl
          Ligand concentration: ~ 25μmol Ligand/mL resin
          Dynamic binding capacity: ~ 45mg α-Chymotrypsinogen /mL packed resin
          Max. pressure: 0.3MPa
          Pressure flow velocity: ≥200cm/h (0.1MPa,BXK 50/30, H=15cm, 25°C)
          Chemical stability: Stable in common aqueous buffers: 1M NaOH++, 1M HAc++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol
          pH stability: 3~13 (CIP), 3~12 (working)
          Operating temperature: Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C high pressure sterilization (20min)
          Storage+++: 2~30°C, 20% ethanol or 2% benzyl alcohol
          Recommended flow velocity: 60-120cm/h

          +Particle size is normally distributed, and particles within this range account for more than 75% of the total.
          ++1M NaOH and 1M HAc only be used for cleaning.
          +++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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