Phenyl Bestarose FF(LS) - Phenyl HIC Resins

Phenyl Bestarose FF(LS) - Phenyl HIC Resins

Catalog Number:
CM01502227BES
Mfr. No.:
AH0021-AH211316
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      • Overview
        • Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
          Phenyl Bestarose FF (LS) is formed by coupling low substitution (Low Sub) hydrophobic Phenyl groups on 6% agarose base matrix. The High physical and chemical stability of agarose base matrix make provides it good pressure flow velocity performance, making it an ideal choice for large-scale purification process. Suitable for the separation and purification of recombinant proteins, antibodies, enzymes, nucleic acids and other biomolecules with low hydrophobicity, especially in the capture or intermediate purification stage requiring high flow velocity separation process.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Phenyl HIC Resins
          Other Properties
          Appearance: White slurry
          Matrix: Highly Cross-linked agarose, 6%
          Functional group: Phenyl
          Ligand concentration: ~ 25μmol Ligand/mL resin
          Particle size+: 45~165μm (the average particle size is 90μm)
          Max. pressure: 0.3MPa
          Pressure flow velocity: 250~400cm/h (0.1MPa BXK50/60, H=25cm, 20°C)
          Chemical stability: Stable in common aqueous buffers: 1M NaOH++, 1M HAc++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol
          pH stability: 2~14 (CIP),3~13 (working)
          Operating temperature: Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C high pressure sterilization (20min)
          Storage+++: 2~30°C, 20% ethanol or 2% benzyl alcohol
          Recommend flow velocity: 150~300cm/h

          +Particle size is normally distributed, and particles within this range account for more than 95% of the total.
          ++1M NaOH and 1M HAc only be used for cleaning.
          +++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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