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Overview
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This is a personalized conjugation kit (PerKit™) developed for labeling any IgG type antibody with small molecules containing carboxylic acid (-COOH) functional groups. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by a reaction with the surface amine groups of the protein to form a stable amide bond. The final product is then purified to remove any unreacted small molecule acid.
This kit provides materials to label 1-3 mg of one IgG type antibody with a small molecule acid.
• Requirement for Small Molecule
Preferably > 90% pure by C18 HPLC
Total amount: 20 µmol
Absence of primary or secondary amine (-NH2) groups
Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
• Requirement for Antibody (IgG)
Preferably > 90% pure by gel electrophoresis
Total amount: 1-3 mg protein content as measured by UV
• Key Features of this PerKit™
Offers a simple and easy way to label IgG with any small molecule containing carboxylic acid
Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
Offers in-process optimization of the degree of loading during the conjugation
All reagents and supplies included for preparation and purification
Over 90% pure conjugated products by SECPlease contact us at for specific academic pricing.
More Details
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- Properties
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Overview