PerfectStart® IV Fast Probe qPCR SuperMix UDG

PerfectStart® IV Fast Probe qPCR SuperMix UDG is supplied at a 2× concentration, and is a pre-mix reagent for the amplification and detection of DNA in probe-based qPCR. Simply add primers, probe and template to perform singleplex, duplex and multiplex PCR reactions in one reaction well. This product contains newly upgraded PerfectStart® IV Taq Hot-Start DNA Polymerase (Genetically modified next generation Taq DNA polymerase, using antibody blocking method to effectively block the activity of DNA polymerase to prevent non-specific amplification at low temperature), combined with optimal reaction buffer, which significantly improves template affinity, qPCR amplification performance and the sensitivity of low-concentration template detection. It has strong resistance to impurity samples, which is suitable for a variety of detection scene. This product can support fast amplification, enabling 45 cycles of PCR amplification to be completed in 35 minutes. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and ensure the accuracy of results.

Features
• Hot start Taq DNA Polymerase blocking by antibodies, enabling high specificity, high sensitivity, high amplification efficiency, and a wide range of applicable species.
• High-efficiency antibody blocking supports full premixing of primers and probes, high anti-inhibition ability. This reagent can be used for direct amplication from blood, tissue homogenate, swab and other samples.
• Supports fast amplification, enabling 45 cycles of PCR amplification to be completed in 35 minutes.
• The dUTP/UDG anti-contamination system is used to effectively prevent carry-over contamination of PCR products, ensuring the accuracy of results.
• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to pipetting errors

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