PeakV™ Integrated Automated qPCR Instrument

PeakV is a cutting-edge, full-range multiplexing, fully automated sample-to- answer qPCR instrument. With no need for user liquid or sample handling, PeakV offers sample preparation (RNA/DNA magnetic bead extraction/purification), open PCR or panel assay capabilities, and sophisticated multiple-site nested PCR and pre- amplification. The sample-to-answer turnaround time is less than 30 minutes. Key specifications of the PeakV instrument include:
· Sample-to-answer system running on an Open-assay Cartridge of single target to large plex panel tests (World's First):
Easily run any third-party lab-based fluorescent probe PCR assay on PeakV to achieve sample-to-answer testing with minimum assay adaptation effort for ready conversion of manual tests to auto test on PeakV.
· Contamination-free:
The fully sealed cartridge technology, closed-cap processing of sample tubes, and minimal or no user manual handling of sample liquid ensure a contamination-free operation.
· Hands-free operation:
A user directly loads a sample tube containing sample into a cartridge or instrument without liquid handling or opening a cap on the sample tube by the user. The rest is a fully automated process in PeakV, including sample transfer from a sample tube to the cartridge, magnetic bead sample preparation, pre-amplification, and PCR panel results. Since no sample handling is involved, a user can run a test without reading a user manual.
· Ultra-sensitive detection:
Integrated multiple-site nested PCR (MS-nPCR) or multiple-site pre-amplification (MS-preAmp) provide significantly higher sensitivity and specificity in PCR tests compared to traditional qPCR devices. MS-nPCR and MS-preAmp enable much more uniform PCR enrichment of targets in multiple PCR tubes during the first round of nested PCR or preAmp step. These automated steps make PeakV the ideal device for low copy detection applications, such as cancer testing and infectious disease testing.
· Superfast turnaround time:
Since there is no user hands-on steps, one can obtain sample-to-answer results in less than 30 minutes for most sample types. With direct PCR or extraction-free testing protocols, turnaround time can be as little as 10 minutes.
· Flexible multiplexing with full panel size coverage:
A user can assemble a PCR panel at ease to perform single target tests, small and medium panel tests, and a large syndromic panel tests of up to 64 pathogen targets (16 tubes in the panel) in a single run.
· Sample versatility:
Process most sample types, including swabs, saliva, sputum, blood, feces, urine, and tissue with minimal hands-on steps by the user.
· Superfast qPCR:
Complete 40 thermal cycles in as little as 3 minutes and a sensitive qPCR and RT-qPCR detection in less than 7 minutes.
· Large sample volume:
Accommodate 1mL samples, upgradable to 5mL.
· Integrable to a centrifugation workflow:
Seamless integration of a centrifugation step into PeakV user workflow without manual liquid handling of sample or reagent tubes by the user.
· Integrable to a bead beating workflow:
Seamless integration of bead beating step into PeakV user workflow without manual liquid handling of sample or reagent tubes by the user.
· Low cartridge cost:
The innovative cartridge design enables simple and efficient manufacturing processes, leading to low-cost and reliable cartridge production, as compared to regular microfluidics cartridge production processes.
· High cartridge operation reliability:
The straightforward cartridge design, free from microchannels, microvalves, and microchambers, ensures dependable operation.
· Random access with multiple sample processing on PeakV4 and PeakV8 instrument options:
Upgradeable between 4 and 8 sample random access testing capabilities.
· Open PCR panel design:
Easily insert third-party PCR assays or LDT assays into PeakV cartridges for new test panels.
· Operating system:
Microsoft Windows 10/11 or later