pAAVdual-EF1a-Rluc

pAAVdual-EF1a-Rluc is used to produce AAV-EF1a-Rluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a EF1a promoter and an Rluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-EF1a-Rluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The EF1a promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
Renilla luciferase (Rluc) is a bioluminescent reporter protein derived from the sea pansy Renilla reniformis. Rluc catalyzes the oxidation of its substrate, coelenterazine, to produce light, typically in the blue to green range. This property makes Rluc a widely used tool in biological research, particularly for real-time imaging and monitoring of cellular processes. AAV-Rluc is a valuable tool for researchers who need to perform sensitive and non-invasive imaging and monitoring of gene expression and biological processes. Its high sensitivity, low background, and versatility make it particularly useful for in vivo studies, dual-reporter assays, and real-time tracking of cellular events.

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