pAAVdual-EF1a-Gluc

pAAVdual-EF1a-Gluc is used to produce AAV-EF1a-Gluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a EF1a promoter and an Gluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-EF1a-Gluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The EF1a promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
Gaussia luciferase (Gluc) is a highly sensitive bioluminescent reporter protein commonly used in biological research to monitor gene expression, track cellular processes, or assess promoter activity in real time. By employing Gluc as a reporter, researchers gain the ability to effortlessly quantify the transduction efficiency of AAV vectors within target cells. The AAV-Gluc system allows researchers to perform in vivo and in vitro bioluminescent assays.

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