pAAVdual-CaMKIIα(0.36)-Rluc

pAAVdual-CaMKIIα(0.36)-Rluc is used to produce AAV-CaMKIIα(0.36)-Rluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα(0.36) promoter and an Rluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα(0.36)-Rluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.
Renilla luciferase (Rluc) is a bioluminescent reporter protein derived from the sea pansy Renilla reniformis. Rluc catalyzes the oxidation of its substrate, coelenterazine, to produce light, typically in the blue to green range. This property makes Rluc a widely used tool in biological research, particularly for real-time imaging and monitoring of cellular processes. AAV-Rluc is a valuable tool for researchers who need to perform sensitive and non-invasive imaging and monitoring of gene expression and biological processes. Its high sensitivity, low background, and versatility make it particularly useful for in vivo studies, dual-reporter assays, and real-time tracking of cellular events.

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