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Figure 1. Immunohistochemical identification of Neurotensin Receptor 2 in gastric mucosa. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NTS2 (Neurotensin receptor 2) antibody (7TM0310N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NTS2 receptors were uniformly detected at the plasma membrane of parietal cells in the gastric muscosa.

Figure 2. Immunohistochemical identification of Neurotensin Receptor 2 in exocrine pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NTS2 (Neurotensin receptor 2) antibody (77TM0310N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NTS2 receptors were detected predominantly at the level of plasma membrane of cells in exocrine pancreas.

Figure 3. Immunocytochemical identification of Neurotensin Receptor 2 in HEK293 cells. HEK293 cells stably expressing the Neurotensin Receptor 2 (NTS2) were either not exposed or exposed to 1 μM Neurotensin for 30 min and immunocytochemically stained with anti-NTS2 (Neurotensin receptor 2) antibody (7TM0310N-IC) at a dilution of 1:200. Note, NTS2 receptors were confined to the plasma membrane in untreated cells (0 min). NTS2 receptors were seen to be translocated to cytosol after 30 min Neurotensin exposure.

Figure 4. Validation of the Neurotensin receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Neurotensin Receptor 2 (NTS2) were lysed and immunoblotted with the anti-NTS2 antibody (7TM0310N-IC) at a dilution of 1:1000.

NTS2 ( IHC-grade ), Neurotensin Receptor 2 Polyclonal Antibody

NTS2 ( IHC-grade ), Neurotensin Receptor 2 Polyclonal Antibody

Catalog Number:

A001456020TMA

Mfr. No.:

7TM0310N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The NTS2 antibody is directed against the distal end of the carboxyl-terminal tail of human Neurotensin Receptor 2. It can be used to detect total NTS2 receptors in Western blots independent of phosphorylation. The NTS2 antibody can also be used to isolate and enrich NTS2 receptors from cell and tissue lysates. It also detects NTS2 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide with sequence PQSPTLMDTASGFGDPPETRT corresponds to amino acids 390-410 of human NTS2.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  NTS2, NTSR2, Neurotensin Receptor 2 (UniProt:O95665)
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Röcken C, Ebert MP, Schulz S. Immunocytochemical identification of low-affinity NTS2 neurotensin receptors in parietal cells of human gastric mucosa. J Endocrinol. 2006 Oct;191(1):121-8. PubMed PMID: 17065395.

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