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Figure 1. Immunohistochemical Identification of Serine594/Threonine597/Serine599 phosphorylation of the SMO Receptor in primary cilia in the mouse neural tube. Neural tubes from wild-type E9.5 mice were stained for pS594/pT597/pS599-SMO antibody and Arl13b. Higher magnification images were acquired in the ventral neural tube region, the area with the highest activation of the Hh pathway.

Figure 2. Immunocytochemical identification of phospho-SMO Receptor in NIH3T3 cells. NIH3T3 cells, stably expressing the Flag-tagged SMO receptor, were either not exposed or exposed to 1 µM SAG21K overnight and immunocytochemically stained with anti-pS594/pT597/pS599-SMO antibody (7TM0239A-IC) at a dilution of 1:1000. Note, phospho-SMO receptors were detected primary clilia only in agonist-treated cells.

Figure 3. Agonist-induced Serine594/Threonine597/Serine599 phosphorylation of the SMO Receptor. Upper panel, NIH3T3 cells stably expressing the Flag-tagged SMO receptor were either not exposed or exposed to 1 µM SAG21K for 4 hours in the absence or presence of 30 µM of the the GRK2/3 inhbibitor Compound 101. Cells were lysed and immunoblotted with anti-pS594/pT597/pS599-SMO antibody (7TM0239A-IC) at a dilution of 1:1000. Lower panel, blot was probed with the phosphorylation-independent anti-FLAG antibody (7TM00FLAG) at a dilution of 1:1000 to confirm equal amounts of total SMO in each sample.

pS594/pT597/pS599-SMO ( phospho-SMO Receptor Polyclonal Antibody )

pS594/pT597/pS599-SMO ( phospho-SMO Receptor Polyclonal Antibody )

Catalog Number:

A001456021TMA

Mfr. No.:

7TM0239A-IC

Price:

$720

Size:

300 µl

Quantity:

Add to Cart:

Overview
- Serine594/Threonine597/Serine599 (S362/S363/S364) is major GRK2 phosphorylation site of the SMO receptor. The pS594/pT597/pS599-SMO antibody detects phosphorylation in response to agonists. The pS594/pT597/pS599-SMO antibody can be used for detection of the subcellular location of phosphorylated SMO by immunocytochemistry or by immunohistochemistry in mouse brain.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic phosphopeptide derived from mouse SMO around the phosphorylation site of Ser594/Thr597/Ser599.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  SMOH | FZD11 | frizzled family member 11 | bnb | smoothened, frizzled class receptor | smoothened (UniProt:Q99835 (human), P56726 (mouse), P97698 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:1000
Immunohistochemistry (IHC)	1:1000

Reference
- Walker MF, Zhang J, Steiner W, Ku PI, Zhu JF, Michaelson Z, Yen YC, Long AB, Casey MJ, Poddar A, Nelson IB, Arveseth CD, Nagel F, Clough R, LaPotin S, Kwan KM, Schulz S, Stewart RA, Tesmer JJG, Caspary T, Subramanian R, Ge X, Myers BR. A Ciliary SMOOTHENED-GRK2-PKA Signaling Pathway Initiates Hedgehog Signal Transduction. bioRxiv. 2023 May 11:2023.05.10.540226. doi: 10.1101/2023.05.10.540226. PMID: 37214942; PMCID: PMC10197709.

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