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Figure 1. Immunohistochemical control of anti-NPS (Neuropeptide S) antibody in mouse coronal sections. Sections were quenched, blocked and incubated with anti-NPS (Neuropeptide S) antibody (7TM0NPS-IC) at a dilution of 1:100 (left picture) or incubated with the same antibody in the same dilution but antibody was preincubated with 1 µM Neuropeptide S (right picture). Sections were then washed and treated with Alexa Fluor 555-conjugated goat anti-rabbit IgG. Note, NPS staining was abolished in section when antibody was preincubated with NPS peptide.

Figure 2. Immunohistochemical identification of Neuropeptide S (NPS) in mouse telencephalon. Sections were quenched, blocked and incubated with anti-NPS (Neuropeptide S) antibody (7TM0NPS-IC) at a dilution of 1:100. Sections were then washed and treated with Alexa Fluor 555-conjugated goat anti-rabbit IgG. Note, strong NPS signals were detected in the medial part of the medial preoptic nucleus and moderate staining was observed in strial part and anterodorsal part of preoptic area.

Figure 3. Immunohistochemical identification of Neuropeptide S (NPS) in mouse diencephalon. Sections were quenched, blocked and incubated with anti-NPS (Neuropeptide S) antibody (7TM0NPS-IC) at a dilution of 1:100. Sections were then washed and treated with Alexa Fluor 555-conjugated goat anti-rabbit IgG. Note, NPS was detected in anterior part of the paraventricular thalamic nucleus. Right picture show NPS staining around A14 dopamine cells and anterodorsal thalimic nucleus.

NPS ( IHC-grade ), Neuropeptide S Polyclonal Antibody

NPS ( IHC-grade ), Neuropeptide S Polyclonal Antibody

Catalog Number:

A001455989TMA

Mfr. No.:

7TM0NPS-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The NPS antibody is directed against human Neuropeptide S. The NPS antibody can be used to detect Neuropeptide S in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents human NPS.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  NPS, neuropeptide S (UniProt:P0C0P6)
  
  * This product is intended for research use only.

Applications

Application

IHC

Application	Dilutions
Immunohistochemistry (IHC)	1:100

Reference
- Clark SD, Duangdao DM, Schulz S, Zhang L, Xiaobin L, Yang-Lin X, Reinscheid RK. Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry. J Comp Neurol. 2011 Jul 1;519(10):1867-93. doi: 10.1002/cne.22606. PubMed PMID: 21452235.

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