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Figure 1. Immunohistochemical identification of Complement C5a Receptor 1 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (Complement C5a Receptor 1) antibody (7TM0032N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were detected at the plasma membrane of a distinct population of cells in human spleen.

Figure 2. Immunohistochemical identification of Complement C5a Receptor 1 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (Complement C5a Receptor 1) antibody (7TM0032N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were detected at the plasma membrane of a distinct population of cells in human spleen.

Figure 3. Immunohistochemical identification of Complement C5a Receptor 1 in human lung. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (Complement C5a Receptor 1) antibody (7TM00032N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were uniformly detected at the plasma membrane of lung cells.

Figure 4. Immunocytochemical identification of Complement C5a Receptor 1 in HEK293 cells. HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were either not exposed or exposed to 1 μM C5a (complement fragment 5a) for 30 min and immunocytochemically stained with anti-C5a1 (Complement C5a Receptor 1) antibody (7TM0032N-IC) at a dilution of 1:200. Note, C5a1 receptors were confined to the plasma membrane in untreated cells (0 min). C5a1 receptors were seen in perinuclear clusters of vesicles after 30 min C5a exposure.

Figure 5. Validation of the Complement C5a Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were lysed and immunoblotted with the anti-C5a1 antibody (7TM0032N-IC) at a dilution of 1:1000.

C5a1 ( IHC-grade ), Complement C5a Receptor 1 Polyclonal Antibody

C5a1 ( IHC-grade ), Complement C5a Receptor 1 Polyclonal Antibody

Catalog Number:

A001455991TMA

Mfr. No.:

7TM0032N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The C5a1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Complement 5a Receptor 1. It can be used to detect total C5a1 receptors in Western blots independent of phosphorylation. The C5a1 antibody can also be used to isolate and enrich C5a1 receptors from cell and tissue lysates. It also detects C5a1 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents distal end of carboxyl-terminal tail of human C5a1
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin Chemotactic Receptor 1 (UniProt:P21730)
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PLoS One. 2021 Feb 19;16(2):e0246939. doi: 10.1371/journal.pone.0246939. PMID: 33606748; PMCID: PMC7894821.

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