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Figure 1. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR4 (Atypical Chemokine Receptor 4) antibody (7TM0315N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ACKR4 receptors were detected at the plasma membrane of nearly all cells in human spleen.

Figure 2. Immunohistochemical identification of Atypical Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR4 (Atypical Chemokine Receptor 4) antibody (7TM0315N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, ACKR4 receptors were detected at the plasma membrane of nearly all cells in human spleen.

Figure 3. Validation of Atypical Chemokine Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 4 (ACKR4) were lysed and immunoblotted with the anti-ACKR4 antibody (7TM0315N-IC) at a dilution of 1:1000.

ACKR4 ( IHC-grade ), Atypical Chemokine Receptor 4 Polyclonal Antibody

ACKR4 ( IHC-grade ), Atypical Chemokine Receptor 4 Polyclonal Antibody

Catalog Number:

A001455992TMA

Mfr. No.:

7TM0315N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The ACKR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Atypical Chemokine Receptor 4. It can be used to detect total ACKR4 receptors in Western blots independent of phosphorylation. The ACKR4 antibody can also be used to detect ACKR4 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents distal end of carboxyl-terminal tail of human ACKR4
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  ACKR4, Atypical Chemokine Receptor 4 (UniProt:Q9NPB9)
  
  * This product is intended for research use only.

Applications

Application

WB; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100

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