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Figure 1. Immunohistochemical identification of Neuropeptide B (NPB) in rat hypothalamus. Rats were treated with an intracerebroventricular injection with 100 µg colchicine (10µg/µl) followed by vascular perfusion and fixation. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NPB (Neuropeptide B) antibody (7TMNPB-IC) at a dilution of 1:100 or incubated with the same antibody in the same dilution but antibody was preincubated with 10 µg/ml Neuropeptide B (right picture). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation with streptavidine-cyanine 3.18. Note, after colchicine treatement Neuropeptide B was located in neuronal somata. NPB staining was abolished in section when antibody was preincubated with NPB peptide.

Figure 2. Immunohistochemical identification of Neuropeptide B (NPB) in rat hypothalamus. Rats were treated with an intracerebroventricular injection of a vehicle instead of colchicine (Figure 1) followed by vascular perfusion and fixation. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NPB (Neuropeptide B) antibody (7TMNPB-IC) at a dilution of 1:100 or incubated with the same antibody in the same dilution but antibody was preincubated with 10 µg/ml Neuropeptide B (right picture). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation with streptavidine-cyanine 3.18. Note, without colchicine treatment Neuropeptide B was located in fibers and terminals. NPB staining was abolished in section when antibody was preincubated with NPB peptide. .

NPB ( IHC-grade ), Neuropeptide B Polyclonal Antibody

NPB ( IHC-grade ), Neuropeptide B Polyclonal Antibody

Catalog Number:

A001455988TMA

Mfr. No.:

7TM0NPB-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The NPB antibody is directed against human Neuropeptide B. The NPB antibody can be used to detect Neuropeptide B in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic peptide presents human NPB.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  NPB, neuropeptide B (UniProt:Q8NG41)
  
  * This product is intended for research use only.

Applications

Application

IHC

Application	Dilutions
Immunohistochemistry (IHC)	1:100

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