Login / Register

0

Figure 1. Immunohistochemical identification of Nociceptin/Orphanin FQ in rat dorsal horn. Sections were dewaxed, microwaved in citric acid, and incubated with anti-N/OFQ (Nociceptin/Orphanin FQ) antibody (7TMNOFQ-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, Nociceptin/Orphanin FQ was detected in a dense plexus of fibres and terminals in the superficial dorsal horn.

Figure 2. Immunocytochemical analysis of Nociceptin/Orphanin FQ expression in N/OFQ+/+ and N/OFQ-/- mouse brains. Coronal sections of wildtype (N/OFQ +/+) and Nociceptin/Orphanin FQ knockout (N/OFQ/-/-) mice were dewaxed, microwaved in citric acid, and incubated with anti-N/OFQ (Nociceptin/Orphanin FQ) antibody (7TMNOFQ-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, Nociceptin/Orphanin FQ staining was only detected in wildtype mice in mice in the ventromedial hypothalamic nucleus, amygdala, and habenular nucleus.

Figure 3. Immunofluorescent identification of Nociceptin/Orphanin FQ in rat spinal trigeminal nucleus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-N/OFQ (Nociceptin/Orphanin FQ) antibody (7TMNOFQ-IC) at a dilution of 1:100 or an mouse anti-Enkephalin antibody. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation with streptavidine-cyanine 2.18 (green) and anti-mouse-cyanine 5.18 (red). Note, Nociceptin/Orphanin FQ (green) and Enkephalin (red) were distributed in the same tissue but show no co-localization.

N/OFQ ( IHC-grade ), Nociceptin/Orphanin FQ Polyclonal Antibody

N/OFQ ( IHC-grade ), Nociceptin/Orphanin FQ Polyclonal Antibody

Catalog Number:

A001455987TMA

Mfr. No.:

7TMNOFQ-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The N/OFQ receptor antibody is directed against human Nociceptin/Orphanin FQ. The N/OFQ antibody can be used to detect N/OFQ in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic peptide presents human N/OFQ.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  N/OFQ, nociceptin/orphanin FQ (UniProt:Q13519 (human), Q64387 (mouse), Q62923 (rat))
  
  * This product is intended for research use only.

Applications

Application

IHC

Application	Dilutions
Immunohistochemistry (IHC)	1:100

Reference
- Köster A, Montkowski A, Schulz S, Stübe EM, Knaut K, Jenck F, Moreau JL, Nothacker HP, Civelli O, Reinscheid RK. Targeted disruption of the orphanin FQ/nociceptin gene increases stress susceptibility and impairs stress adaptation in mice. Pro Natl Acad Sci USA. 1999 Aug 31;96(18):10444-9. doi: 10.1073/pnas.96.18.10444.
  Schulz S, Schreff M, Nüss D, Gramsch C, Höllt V. Nociceptin/orphan FQ and opioid peptides show overlapping distribution but no co-localization in pain-modulatory brain regions. Neuroreport. 1996 Nov 25;7(18):3021-5.PubMed PMID: 9116232.

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.