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Overview
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Background
Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red.
Reference:
1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.
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- Properties
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Overview