Nerve Organoid™ B-Chip for co-culture target cell types and synapse formation

B-Chip consists of 2 wells connected by a microchannel. The 2 wells are separated by a wall, allowing researchers to culture cells in different media in each well. iPSC-derived neuronal spheres are seeded into the smaller well and axons grow into the microchannel. During culture, axons self-organize into a bundle and extend into the far well where they branch out and expand uniformly into growth cones. B-Chips can be used to co-culture target cell types and to induce human synapse formation using our synaptogenic beads. B-chip fits 48 chips into an SBS standard 96-well plate, making it compatible with imaging systems.

• Chip materials: COP (cyclic olefin polymer; Zeon Corporation, Japan) Transparent, chemical resistant, heat resistant, low protein adsorption, low elution（US Pharmacopeia Class VI compliant）
• Outer dimensions: 17.9 mm (W) x 8.8 mm (D) x 12.4 mm (H)
• Microchannel length: 2.6 mm
• Selling unit: Available individually

Please contact us at for specific academic pricing.

Background

Amerigo Scientific provides proprietary culture chip devices for the culture of Nerve Organoid™. Each chip is made up of a microfluidic channel fabricated using microfabrication technology and is sandwich by culture wells at both ends. When culturing neuron spheroid cell body in one well, axons from the cell body is capable of extending into the channel to form self-organized axon bundles.

Our patented Nerve Organoid™ consists of 3D nerve tissue in a unique microfluidics device. The 3D nerve tissue is derived from human iPS nerve cells. It closely resembles an in vivo nerve with a cell body and axons that self-organize into bundles as they extend through the device’s microchannel.

Nerve Organoid™ features:
• Structurally separate neural cell body and axon bundle for ease of whole organoid, cellular soma, or acellular axon bundle analyses
• Bundled axons, morphologically analogous to in vivo
• Robust enough to be manipulated with forceps
• Suitable for long term culture
• Generated from differentiated human iPS cells; reproducible and highly consistent