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NAD/NADH Ratio Assay Kit (Red Fluorescence)

NAD/NADH Ratio Assay Kit (Red Fluorescence)

Catalog Number:

CFA1518277EEN

Mfr. No.:

CA-N226

Price:

$642

Size:

250 assays in 96-well plates

Quantity:

Add to Cart:

- Overview
  - This Fluorimetric NAD/NADH Ratio Assay Kit provides a convenient method for sensitive detection of NAD, NADH and their ratio; there is no need to purify NAD/NADH from sample mix. It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.
    
    Please contact us at for specific academic pricing.
- Properties
  - * For Research Use Only.
- Applications
  - Application Description
    
    The assay can be conveniently performed in a 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 530 - 570/590 - 600 nm (maximum Ex/Em = 540/590 nm) or an absorbance microplate reader at ~576 nm. This kit provides NAD and NADH extraction buffer, and cell lysis buffer for your convenience. It has been frequently used for determining NAD/NADH from cell lysates.
- Reference
  - 1) Suppressed expression of LDHB promotes age-related hearing loss via aerobic glycolysis. Cell Death Dis. 11, 1–13 (2020).
    2) Identification of the Additional Mitochondrial Liabilities of 2-Hydroxyflutamide When Compared With its Parent Compound, Flutamide in HepG2 Cells. Toxicol. Sci. 153, 341–351 (2016).

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