N3-Methyl Uridine 3'-tBDSilyl-2' lcaa CPG 500A

N-3 Substituted Pyrimidine RNA Amidites and Supports

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

N-3 Substituted Pyrimidine RNA Amidites and Supports:

N3-Methyl-Uridine and N3-Methyl-Thymidine are the results of chemical carcinogens on DNA/RNA. In general, alkylation products are formed at the ring-nitrogen atoms and the exocyclic oxygen atoms of all four bases as well as at the oxygen atoms of the internucleotide phosphate bonds. Such adducts can initiate neoplastic transformations.1
The individual alkylation products are thought to possess different mutagenic and carcinogenic properties.2
N3-Methyl-uridine phosphoramidite and N3-Methyl-thymidine phosphoramidite are currently available for incorporation into RNA/DNA to further study the biochemical nature of these oligonucleotides. It is important to gain an understanding of these ring-nitrogen modified bases for eventual damage repairs.

References:

1. a) Singer, B.; Kusmierek, J. T. Ann. Rev. Biochem. 1982, 52, 522; b) Saffhill, R.; Margison, G. P.; O’Connor, P. J. Biochim. Biophys. Acta 1985, 823, 111.

2. Savov, A.; Angelicheva, D.; Jordanova, A.; Eigel, A.; Kalaydjieva, L. Nucl. Acids Res. 1992, 20, 6471.