N1-Methyl deoxy Adenosine (n-fmoc) 3'-lcaa CPG 1000A

N-Alkylated Amidites and Supports

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

N-Alkylated phosphoramidites and Supports:

DNA in living organisms is vulnerable to alkylation due to mutagenic effects of carcinogens. Recently it has been shown that there is direct reversal of N-alkyl­ation of methylated bases in oligonucleotides. A recent discovery of an enzyme which is a substrate for DNA repair.1
These reports have great implication for repair of such carci­nogenic and mutagenic events.2-7
N-Alkylated nucleosides and the oligonucleotides incorporating methylated bases at specific sites in oligonucleotides possess great potential for studies of reversal of alkylation in oligonucleotides and studies in oxidative alkylation inside cells by various carcinogens.
We are offering the phosphoramidites for studies and possibilities of reversal of methylation lesions by use of oligonucleotides incorporating alkyl­ated purine/pyrimidines.

References:

1. a) Trewick, S. C.; Henshaw, T. F.; Hausinger, R. P.; Lindahl, T.; Sedgwick, B. Nature, 2002, 419, 174-177; b) Falnes, P.; Johansen, R. F.; Seeberg, E. Nature 2002, 419, 178.

2. Delaney, J. C.; Essigmann, J. M. Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 14051-14056.

3. Delaney, J. C. et. al. Nat. Struct. Mol. Biol. 2005, 12, 855-860.

4. Jarosz, D. F. et. al Nature 2006, 439, 225-228.

5. Frick, L. E. et. al. Proc. Natl. Acad. Sci. U.S. A. 2007, 104, 755-760.

6. Lee, C. I. et. al. Biochemistry, 2009, 48, 1850-1861.

7. Jarosz, D. F. et. al. Proc. Natl. Acad. Sci U.S.A. 2009, 106, 21137-21142.