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LC-MS of N-terminal Biotin-Ubiquitin

LC-MS of N-terminal Biotin-Ubiquitin

N-terminal Biotin-Ubiquitin

N-terminal Biotin-Ubiquitin

Catalog Number:

UULP097427UBP

Mfr. No.:

E1140

Price:

$351

Size:

50 µg

Quantity:

Add to Cart:

- Overview
  - A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Biotin-Ub
    
    Source
    
    Synthetic
    
    Formulation
    
    20 mM Tris, pH 7.6 at 4 °C, 100 mM NaCl, 10% glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    1 mg/ml in aqueous buffer
    
    Molecular Weight
    
    8.9 kDa
    
    * Research Use Only

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