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N-terminal Biotin-Ubiquitin

N-terminal Biotin-Ubiquitin

Catalog Number:
UULP097427UBP
Mfr. No.:
E1140
Price:
$351
  • Size:
    50 µg
    Quantity:
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      • Overview

        • A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors.

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      • Properties
        • Protein Name
          Biotin-Ub
          Source
          Synthetic
          Formulation
          20 mM Tris, pH 7.6 at 4 °C, 100 mM NaCl, 10% glycerol
          Storage
          Store at -80°C; avoid multiple freeze-thaw cycles
          Concentration
          1 mg/ml in aqueous buffer
          Molecular Weight
          8.9 kDa

          * Research Use Only

    Datasheet
    MSDS

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