N-Glycan Release & 2-AB Velocity Labelling Module

This 2-AB labelling bundle kit includes PNGase F enzyme for in-solution release of N-glycans, 2-AB labelling kit and clean-up cartridges for post-labelling clean-up. The kit also includes an IgG glycoprotein standard to be used as a process control as well as a glucose homopolymer (system suitability standard). The protocol presented is the golden standard methodology for the analysis of N-linked glycans using (U)HPLC, LC-MS or MALDI-MS approaches.

This bundle kit includes reagents sufficient for the analysis of 30 samples.

Shipping: The product should be shipped at 4°C.

Handling: Ensure that any glass, plasticware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrates.

Safety: For research use only. Not for human or drug use. Please read the Safety Data Sheets (SDSs) for all chemicals used. All processes involving labelling reagents should be performed using appropriate personal safety protection – safety glasses, chemically resistant gloves (e.g. nitrile), lab coat, and when appropriate, in a laboratory fume cupboard.

Application: Release of N-glycans from glycoproteins, subsequent fluorophore labelling and clean up.

Number of Samples: 30 separate analytical samples per kit.

Amount of Sample: As a guideline, up to 50 to 100 μg of glycoprotein per sample for release using PNGase F enzyme.

Suitable Samples: Glycoproteins containing N-linked glycans.

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