Hieff™ Mouse Tissue Direct PCR Kit (With Dye)

Hieff™ Mouse Tissue Direct PCR Kit (With Dye)

Catalog Number:
PK01554113YEA
Mfr. No.:
10185ES
Price:
$230
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      • Overview
        • This kit can directly and quickly conduct PCR amplification of mouse tissue (such as mouse tail, mouse ear, mouse toe, muscle, etc.), and has strong sample compatibility. Equipped with a powerful lysis buffer, this kit can rapidly lyse samples and release genomic DNA. The lysate can be directly added to the PCR reaction system without purification, and the operation is convenient. In addition, this kit requires low sample input, and 5 mg mouse tissue or 1-5 mm mouse tail can be used for experiments.
          The 2× Mouse Direct PCR Mix provided by this kit is a hot-start PCR reaction solution with a 2-fold concentration. It contains all the components used for PCR amplification except the template and primers, which greatly simplifies the operation process and reduces the chance of contamination. The kit can be used for transgene identification, mouse genotyping, etc.

          Features
          • Less sample required: 5 mg of mouse tissue or 1-5 mm of mouse tail.
          • Convenient template preparation: no need to grind, no need to refine and purify DNA, higher flux, time and money saving.
          • Optimized PCR system: with higher specificity and stronger tolerance of PCR reaction inhibitor.

          Please contact us at for specific academic pricing.

      • Properties
        • Storage
          1. Component A: The product should be stored at 2-8°C for one year. For multiple use for a long time, please avoid cross-contamination.
          2. Component B/C: The product should be stored at -25 ~ -15°C for one year. Please avoid repeated freeze-thaw.

          * For Research Use Only.

      • Applications
        • Application Description
          Identification of transgenic mice
          Genotyping of mice
          Mouse gene knockout analysis
      • Reference
        • [1] Luo J, Yang Q, Zhang X, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016/j.cell.2022.02.010(IF:41.584)
          [2] Zhao J, Chen J, Li YY, Xia LL, Wu YG. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48(3):177. doi:10.3892/ijmm.2021.5010(IF:4.101)

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