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Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Cells

Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Cells

Catalog Number:
SC01279068APP
Mfr. No.:
T2028
Price:
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  • Size:
    1x10⁶ cells/ml
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      • Overview

        • Supporting cells, such as short-term cultures of fibroblasts, are used to cultivate human embryonic stem (ES) cells, however they are susceptible to senescence after a number of passages. The Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Cells eliminates the problems associated with the use of primary cell lines. This cell line has a doubling time of 16 hours and may be used indefinitely to propagate human embryonic stem cells. It has the ability to facilitate the growth of hES H9 and hES H1. MMMbz cells are resistant to blasticidin and zeocin due to transgene expression allowing for selection of hES cells transfected with genes conferring resistance.

          Please contact us at for specific academic pricing.

      • Properties
        • Source
          Mouse (M. musculus)
          Type
          Feeder Cells
          Storage
          -180°C
          Origin
          Tissue: Reproductive

          * For research use only. Not for diagnostic or therapeutic use.

      • Reference

        • Iuchi, S et al. "An immortalized drug-resistant cell line established from 12-13-day mouse embryos for the propagation of human embryonic stem cells." Differentiation 74(4):160-166 (2006). PubMed: 16683986.
          Subramanian, V et al. "H2A.Z Acidic Patch Couples Chromatin Dynamics to Regulation of Gene Expression Programs during ESC Differentiation" PLoS Genet. 9(8):e1003725 (2013). DOI: 10.1371/journal.pgen.1003725.

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