Mouse anti Human IgG IgA IgM IgD IgE (class specific)

Mouse anti Human IgG IgA IgM IgD IgE (class specific)

Catalog Number:
A001006709NOR
Mfr. No.:
MAHu/Ig Set
Price:
$918
  • Size:
    5x0.5 mg
    Quantity:
    Add to Cart:
      • Overview
        • Delipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added. The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.

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          Background

          The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.

      • Properties
        • Host
          mouse
          Isotype
          IgG1
          Clone
          Mixed
          Formulation
          Delipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added.
          Storage
          Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day. Monoclonal antibodies should not be stored at a temperature below -25°C due to the aggregation effect of the protein.
          Note
          This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

          * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

      • Applications
        • Application Description
          Indirect immunofluorescence, ELISA.

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