Name: Mouse anti Human IgG (class specific)
Price: 473.00 USD

- Overview
  - Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1
    
    Clone
    
    NI 335 and NI 343
    
    Formulation
    
    Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1.0 mg/ml. No foreign proteins added.
    
    Storage
    
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application Description
    
    Indirect immunofluorescence, ELISA.

Datasheet

Mouse anti Human IgG (class specific)

Mouse anti Human IgG (class specific)

Background

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