Monkey IFN-γ T Cell ELISPOT Kit

Monkey IFN-γ T Cell ELISPOT Kit

Catalog Number:
EK02494176UCY
Mfr. No.:
CT121-PR2/PR5
Price:
$754
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      • Overview
        • The T cell ELISPOT assay is a simple and sensitive immunoassay for the detection of protein secreting T cells at the single cell level.

          Staining: Enzymatic
          Characteristics: PVDF

          Specificity:
          Rhesus macaque
          Cynomolgus monkey
          Pig-tailed macaque
          Baboon
          African green monkey

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          • Coating antibodies
          • Biotinylated detection antibodies
          • Streptavidin-Horseradish Peroxidase
          • AEC coloring system
          • Blocking stock solution
          • Dilution buffer R
          • Tween-20
          • Detailed manual

          * For research use only.

      • Applications
        • Application Description
          The ELISPOT assay is one of the most sensitive assays to monitor ex vivo cellular immune responses at the single cell level. The assay can accurately detect secreted proteins, such as cytokines, released by T cells in response to an antigen. The cell suspensions used in the test can be from blood (PBMC), lymphoid, spleen, bone marrow or CNS tissue.
      • Reference
        • • Host Immune Responses after Suprachoroidal Delivery of AAV8 in Nonhuman Primate Eyes.
          Chung, Sook Hyun, et al. Hum Gene Ther 2021 Jul;32: 682-693
          • SIV Infection Facilitates Mycobacterium tuberculosis Infection of Rhesus Macaques.
          Guo, Ming, et al. Front Microbiol 2017;7: 2174
          • Use of an Sm-p80-based therapeutic vaccine to kill established adult schistosome parasites in chronically infected baboons.
          Karmakar, Souvik, et al. J. Infect. Dis. 2014 Jun 15;209(12): 1929-40
          • HIV-1 vaccines based on replication-competent Tiantan vaccinia protected Chinese rhesus macaques from simian HIV infection.
          Liu, Qiang, et al. AIDS 2015 Mar 27;29: 649-58
          • Poxvirus MVA Expressing SARS-CoV-2 S Protein Induces Robust Immunity and Protects Rhesus Macaques From SARS-CoV-2.
          Mooij, Petra, et al. Front Immunol 2022;13: 845887
          • Vaccine-induced neutralizing antibody responses to seasonal influenza virus H1N1 strains are not enhanced during subsequent pandemic H1N1 infection.
          Mooij, Petra, et al. Front Immunol 2023;14: 1256094
          • No difference in Gag and Env immune-response profiles between vaccinated and non-vaccinated rhesus macaques that control immunodeficiency virus replication.
          Nieuwenhuis, Ivonne, et al. J. Gen. Virol. 2010 Dec;91: 2974-84
          • T- and B-cell responses to multivalent prime-boost DNA and viral vectored vaccine combinations against hepatitis C virus in non-human primates.
          Rollier, C S, et al. Gene Ther. 2016 10;23(10): 753-759
          • A Next-Generation Adenoviral Vaccine Elicits Mucosal and Systemic Immunogenicity and Reduces Viral Shedding after SARS-CoV-2 Challenge in Nonhuman Primates.
          Tedjakusuma, Sarah N, et al. Vaccines 2024 Jan 27;12(2): 132
          • Vaccine-induced protection of rhesus macaques against plasma viremia after intradermal infection with a European lineage 1 strain of West Nile virus.
          Verstrepen, Babs E, et al. PLoS ONE 2014;9: e112568
          • Protective Immune Responses Elicited by Deglycosylated Live-Attenuated Simian Immunodeficiency Virus Vaccine Are Associated with IL-15 Effector Functions.
          Watanabe, Satoru, et al. J Immunol 2020 09 01;205: 1331-1344

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