MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Rat (R. norvegicus)
    
    Type
    
    Feeder Cells
    
    Storage
    
    -180°C
    
    Origin
    
    Tissue: Reproductive
    
    * For research use only. Not for diagnostic or therapeutic use.
- Applications
  - Application Description
    
    The inactivated REFs have been tested to be significantly better than inactivated MEF in supporting human iPSC reprogramming and long-term undifferentiated proliferation of human iPS cells and human ground state naive pluripotent stem cells. There is no need to change medium at weekend if human iPS or ES cells are split on Friday and cultured on this REF feeder line in iPSC Culture Medium (TM020).
- Reference
  - Linta, L et al. "Rat embryonic fibroblasts improve reprogramming of human keratinocytes into induced pluripotent stem cells" Stem Cells Dev 21(6):965-976 (2012). DOI: 10.1089/scd.2011.0026.
    Su, RJ et al. "Efficient Generation of Integration-Free iPS Cells from Human Adult Peripheral Blood using BCL-XL Together with Yamanaka Factors" PLoS ONE 8(5):e64496 (2013). DOI: 10.1371/journal.pone.0064496.
    Su, RJ et al. "Few Single Nucleotide Variations in Exomes of Human Cord Blood Induced Pluripotent Stem Cells" PloS one 4:e59908 (2013). DOI: 10.1371/journal.pone.0059908 . Application: Generation of iPSC.
    Baylink, D.J. et al. "Vectors and methods for the efficient generation of integration/transgene-free induced pluripotent stem cells from peripheral blood cells" Patent : (2014). PubMed: US20140134143.

MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells

MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells

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