MDB

- Overview
  - Phenylenediamine (OPD) reacts with α-keto acids to form highly fluorescent quinoxaline derivatives. MDB is an o-phenylenediamine analogue. It is the best labeling reagent for α-keto acids because of its reactivity and sensitivity. In HPLC analysis, more than ten different α-keto acids, such as α-keto glutaric acid, pyruvic acid, and phydroxyphenyl-pyruvic acid, can be separately detected at a time, even at concentrations of only a few femtomoles per injection. The sensitivity of MDB is almost 150 times as high as OPD. The excitation and emission wavelength of the labeled materials are 367 nm and 445 nm, respectively.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    HPLC Derivertization Reagents
    
    CAS Number
    
    81864-15-5
    
    Molecular Formula
    
    C7H10Cl2N2O2
    
    Molecular Weight
    
    225.07
    
    Appearance
    
    White or slightly pink crystalline powder
    
    Purity
    
    ≥98.0% (Titration)
    
    Storage
    
    -20°C, protect from moisture
    
    Shipping
    
    with blue ice
    
    * For research use only
- Reference
  - 1) M. Nakamura, et al., 1, 2-Diamino-4, 5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Keto Acids. Chem Pharm Bull. 1987;35:687-692.
    
    2) S. Hara, et al., Fluorometric High-Performance Liquid Chromatography of N-Acetyl-and N-Glycolylneuraminic Acids and Its Application to Their Microdetermination in Human and Animal Sera, Glycoproteins, and Glycolipids. Anal Biochem. 1987;164:138-145.
    
    3) S. Hara, et al., 1, 2-Diamino-4, 5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Dicarbonyl Compounds. Anal Chim Acta. 1988;215:267-276.

MSDS

MDB

We Also Recommend

ABD-F