MAL-dPEG®₃₆-NHS ester

MAL-dPEG®36-NHS ester, product number 10904, crosslinks sulfhydryl groups to free amines. Free thiols react with maleimide groups via a Michael addition reaction. At the same time, amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a long, flexible, discrete-length polyethylene glycol chain (dPEG®).

The most popular, most useful crosslinking reactions in bioconjugate chemistry are those that join free thiols to free amines using heterobifunctional reagents that bridge the two groups. Traditional crosslinkers are hydrophobic molecules, and the conjugation of conventional hydrophobic crosslinking reagents to biomolecules almost inevitably triggers problems such as aggregation and precipitation of the conjugates. By contrast, our dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths. Because of our dPEG® products' hydrophilicity, these problems do not occur when using our crosslinkers on biomolecules.

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