MagPure Blood RNA Kit

MagPure Blood RNA Kit

Catalog Number:
NAPK357166ANG
Mfr. No.:
R661101; R661102; R661103
Price:
$347
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      • Overview
        • This product supplies a simple and rapid extraction of total RNA from Blood, buffy coat, bone marrow, cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on.

          Principle
          The Kit can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5x106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.

          Advantages
          • High purity - OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
          • Economy - less than 50% of the price of Qiagen and other imported products
          • Automatic - extraction without manual participation, saving time and effort
          • High yield - nucleic acid recovery up to 95%
          • General - samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Polydisperse magnetic beads
          Purification technology: Magnetic beads technology
          Process method: Manual or automatic
          Sample type: Whole blood, buffy coat, bone marrow, cultured cells, lymphocytes, body fluid
          Sample amount: ≤5x10^6 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow
          Storage
          Proteinase K, DNase I, MagPure RNA Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

          * For Research Use Only

      • Applications
        • Application
          RT-PCR, cDNA synthesis, second generation sequencing
          Application Description
          Isolation total RNA from 300μl whole blood, bone marrow, buffy coat

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