Luciferase assay Kit

For reporter gene assay allowing the monitoring of promoter activity in the control of gene expression.
484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:

1) Firefly luciferase is not present in normal cells.

2) The assay is simple and easy to perform.

3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.

4) It is suitable for HTS.

The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.

▶ Intrusments
The stable light makes it possible to use manual single tube luminometers as well as microplate luminometers. The detection limit obviously depends on the luminometer. With most luminometers 10^-19 moles of luciferase can be detected.

▶ Assay Procedure
Detailed described procedures are found in the "Instructions for Use", which is included with every kit.

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