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Overview
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Livecyte™ utilises ptychography to capture relative phase shift information, allowing high contrast images to be generated using low level illumination. Individual cells can be identified and characterised according to morphological and behavioural characteristics, providing accurate data for quantitative analysis.
Ptychography works equally well in transmitted light and reflected light applications and, given suitable illumination sources and detectors, it can operate using any wavelength in the electromagnetic spectrum, as well electron and other particle waves and even sound waves.
Our flagship product, Livecyte, uses our powerful imaging techology to produce high-contrast videos of live cells without the need for fluorescent labels or high-power illumination. This makes it possible to monitor even the most sensitive live cells, such as stem cells and heterogeneous primary cancer cells, for long periods of time (up to multiple weeks) in a 96-well plate format.
Livecyte produces exceptionally high contrast time-lapse videos using Ptychographic quantitative phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.
Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.
Easy-to-use Dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.
Product Characteristics
· A label-free high-content system
Livecyte produces exceptionally high contrast time-lapse videos using Phasefocus's patented Ptychographic quantitative phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.
· From Images to Insights
Easy-to-use image processing and tracking algorithms allow individual cells to be identified in real time.
Explore how the properties of those identified cells, and how they change over time.
· Powerful Integrated Analysis
Integrated analysis suite enables cell behaviour to be monitored as a function of treatment.
Easily export results in Excel and Graphpad formats
Monitor many different types of behaviour, from morphological to motile, in every experiment making laboratory time more productive.
· Assay-Level Analysis
Large fields of view (up to 4mm²) in an assay relevant plate format (up to 96 well plate) mean the behaviour of many cells can be captured making results statistically robust.
Unlike other QPI systems, Livecyte's ptychography technology removes the need for plate inserts or expensive consumables - reducing running costs compared to comparative systems.
Correlate changes in proliferation, motility and morphology as a function of treatment, from every experiment.
· Explore Population Heterogeneity
Identify, track and classify individuals and monitor their phenotypic behaviour over time.
Classify subpopulations based of any combination of parameters.
Compare subpopulation behaviour as a function of treatment.
· Single-cell Lineage
Automatically segment and track individuals and identify their division events and children.
Quick and easy track correction enables perfect lineage determination.
Explore single cell or whole lineage phenotypical properties across multiple generations.Please contact us at for specific academic pricing.
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- Properties
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Overview