Lipi-Green

Lipid Droplet Staining
▪ High selectivity to lipid droplet (low background)
▪ 4 color available (blue, green, red, and deep red)
▪ High intracellular retentivity
▪ Applicable to both live and fixed cells

Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). LDs were originally thought to serve as a lipid storage unit, until a recent study showing that LDs play an important role in regulating lipid metabolism, autophagy2) and cellular senescence3). Therefore, LDs have gained great attention as an important tool to elucidate the mechanisms of their formation, growth, fusion, and retraction.

1) T. Fujimoto et al., “Lipid droplets: a classic organelle with new outfits.” Histochem Cell Biol., 2008, 130(2), 263.
2) R. Singh et al., “Autophagy regulates lipid metabolism.” Nature, 2009, 458(7242), 1131.
3) M. Yokoyama et al., “Inhibition of endothelial p53 improves metabolic abnormalities related to dietary obesity.” Cell Reports, 2014, 7(5), 1691.

For more information on Lipi-series and examples, please refer to the publication below:

4) Tatenaka, Y. et al., “Monitoring Lipid Droplet Dynamics in Living Cells by Using Fluorescent Probes” Biochemistry.“, 2019, 58(6), 499-503.

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