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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman COLCA1About TargetGene symbol: COLCA1
Synonyms: C11orf92 CASC12 LOH11CR1F
Species: HUMAN
Gene ID: 399948
Summary: This gene encodes a transmembrane protein that localizes to granular structures, including crystalloid eosinophilic granules and other granular organelles. This gene, along with an overlapping opposite strand gene, has been implicated as a susceptibility locus for colorectal cancer. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001302644:2:1 NM_001302645:2:1 NM_207429:2:1 NM_001302646:2:1 NM_001302647:2:1 NM_001302648:2:1
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- Properties
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