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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ARHGEF9About TargetGene symbol: ARHGEF9
Synonyms: COLLYBISTIN EIEE8 HPEM-2 PEM-2 PEM2
Species: HUMAN
Gene ID: 23229
Summary: The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. This brain-specific protein also acts as an adaptor protein for the recruitment of gephyrin and together these proteins facilitate receceptor recruitement in GABAnergic and glycinergic synapses. Defects in this gene are the cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy, as well as several other types of cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_015185:5:5 NM_001173479:4:4 NM_001173480:4:3 NM_001330495:6:4 NM_001353921:5:5 NM_001353922:5:5 NM_001353923:5:5 NM_001353924:4:4 NM_001353926:4:4 NM_001353927:5:4 NM_001353928:5:5 NM_001369030:6:5 NM_001369031:6:5 NM_001369032:5:5 NM_001369033:5:4 NM_001369034:6:4 NM_001369035:5:4 NM_001369036:6:4 NM_001369037:7:4 NM_001369038:5:4 NM_001369039:5:4 NM_001369040:5:4 NM_001369041:5:4 NM_001369042:6:3 NM_001369043:6:4 NM_001369044:5:4 NM_001369045:8:2
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- Properties
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Overview