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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ARHGEF7About TargetGene symbol: ARHGEF7
Synonyms: BETA-PIX COOL-1 COOL1 Nbla10314 P50 P50BP P85 P85COOL1 P85SPR PAK3 PIXB
Species: HUMAN
Gene ID: 8874
Summary: This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_003899:8:5 NM_145735:9:9 NM_001113511:10:10 NM_001113512:8:8 NM_001113513:8:5 NM_001320851:7:5 NM_001320852:9:9 NM_001320853:8:8 NM_001320854:4:4 NM_001330597:9:5 NM_001330598:7:5 NM_001354046:9:9 NM_001354047:7:7 NM_001354048:9:5 NM_001354049:9:5 NM_001354050:8:5 NM_001354051:8:5 NM_001354052:9:5 NM_001354053:7:5 NM_001354054:9:5 NM_001354055:4:4 NM_001354056:7:5 NM_001354057:9:5 NM_001354058:10:5 NM_001354059:10:5 NM_001354060:8:5 NM_001354061:5:3
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- Properties
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Overview