LegiSeq™ Universal Short Adapters & UDI Primers

The LegiSeq™ Universal Short Adapter is a truncated adapter for Illumina® NGS. The adapters are TA-ligated to library fragments following an A-tailing step and are used with the LegiSeq™ UDI Primers to incorporate Unique (10 nucleotide) Indexes via a subsequent PCR step.

• Produced under optimized NGS Production and Handling Workflows
* Minimising Cross Contamination & Index Hopping risk
* Highest quality Oligos -optimized for NGS
* High ligation efficiency, low adapter-dimer formation
• NGS Quality Control check performed on every production batch
* Ensuring Cross Contamination levels <0.01%
• Compatible with 2 & 4 Colour Illumina® Sequencing Platforms
• Indexes are designed with an edit distance ≥3 (Hamming distance)
• Cross referenced for no duplicate indexes across different Biolegio Index sets and no conflict against index sets from other main suppliers and library preparation kit manufacturers
• All manufactured under a ISO9001:2015 & ISO13485:2016 Accredited Quality Management System

Universal Short Adapter and unique dual indexing primers provided together- For Illumina® sequencing TA-ligation based library preparation (TruSeq™ Compatible). Available as ready-to-use, single-use plates of 16 or 96 UDI primer pairs (10nt Indexes) and an accompanying tube of Universal Short Adapters with a corresponding number of reactions.

Please contact us at for specific academic pricing.