Lactose Assay Kit-Sequential/High Sensitivity

- Overview
  - The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
    
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- Properties
  - Details
    
    Limit of Detection: 1.62mg/L
    
    Storage
    
    Short term stability: 2-8°C
    Long term stability: See individual component labels
    
    Stability
    
    > 2 years under recommended storage conditions
    
    * For research use only.
- Reference
  - 1.Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients by High Sensitivity Enzymatic Method (K-LOLAC), Collaborative Study: Final Action 2020.08. Ivory, R., Mangan, D. & McCleary, B. V. (2022). Journal of AOAC International, qsac070.
    2.Determination of Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients: Single Laboratory Validation of an Enzymatic Method, First Action Method 2020.08. Ivory, R., Delaney, E., Mangan, D. & McCleary, B. V. (2021). Journal of AOAC International, qsab032.
    3.A novel enzymatic method for the measurement of lactose in lactose‐free products. Mangan, D., McCleary, B. V., Culleton, H., Cornaggia, C., Ivory, R., McKie, V. A., Delaney, E. & Kargelis, T. (2018). Journal of the Science of Food and Agriculture, 99, 947-956.
    4.Film-shaped reusable smart polymer to produce lactose-free milk by simple immersion. Vallejo-García, J. L., Arnaiz, A., Busto, M. D., García, J. M. & Vallejos, S. (2023). European Polymer Journal, 200, 112495.
    5.Effects of lactose hydrolysis and milk type on the quality of lactose-free yoghurt. Popescu, L., Bulgaru, V. & Siminiuc, R. (2022). Journal of Engineering Sciences, (4), 164-175.
    6.Blood neutrophil extracellular traps: a novel target for the assessment of mammary health in transition dairy cows. Jiang, L., Sun, H., Gu, F., He, J., Zhao, F. & Liu, J. (2022). Journal of Animal Science and Biotechnology, 13(1), 1-12.
    7.Effect of Temperature, pH and Amount of Enzyme Used in the Lactose Hydrolysis of Milk. Popescu, L., Bulgaru, V. & Siminiuc, R. (2021). Food and Nutrition Sciences, 12(12), 1243-1254.
    8.Fermentation Ability of Bovine Colostrum by Different Probiotic Strains. Hyrslova, I., Krausova, G., Michlova, T., Kana, A. & Curda, L. (2020). Fermentation, 6(3), 93.
    9.Exploring the potential of microalgae in the recycling of dairy wastes. Gramegna, G., Scortica, A., Scafati, V., Ferella, F., Gurrieri, L., Giovannoni, M., Bassi, R., Sparla, F., Mattei, B. & Benedetti, M. (2020). Bioresource Technology Reports, 12, 100604.
    10.GRP78 regulates milk biosynthesis and the proliferation of bovinemammaryepithelial cells through the mTOR signaling pathway. Liu, Y., Wang, X., Zhen, Z., Yu, Y., Qiu, Y. & Xiang, W. (2019). Cellular & molecular biology letters, 24(1), 1-12.
    11.Comparative study of two lactases by K-Lolac enzymatic method in skimmed milk. Benbouziane, B., Bentahar, M. C., Takarly, H. & Benakriche, B. M. (2019). South Asian Journal of Experimental Biology, 9(1), 1-6.
    12.Lactose-free Yogurts do not Show any Benefits for Lactose-Intolerant Subjects, Compared with Lactose-Containing Yogurts. Ghio, B., Márquez, D., Peche, B., Peña, F. & Saavedra, F. (2019). Journal of Food & Nutritional Disorders, 8(3), 2.
    13.Influence of particle size on the physicochemical properties and stickiness of dairy powders. O'Donoghue, L. T., Haque, M. K., Kennedy, D., Laffir, F. R., Hogan, S. A., O'Mahony, J. A. & Murphy, E. G. (2019). International Dairy Journal, 98, 54-63.
    14.Accurate analysis of residual lactose in low-lactose milk: Comparing a variety of analytical techniques. Churakova, E., Peri, K., Vis, J. S., Smith, D. W., Beam, J. M., Vijverberg, M. P., Stor, M. C. & Winter, R. T. (2019). International Dairy Journal, 96, 126-131.

MSDS

Lactose Assay Kit-Sequential/High Sensitivity

Lactose Assay Kit-Sequential/High Sensitivity

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