Name: Kazusamycin A
Price: 523.00 USD

- Overview
  - Kazusamycin A is a minor member of the Leptomycin complex isolated from some Streptomyces species. It is a hydroxylated analog of Leptomycin B, a nuclear export inhibitor. It is similar to Leptomycins A and B in physico-chemical and biological characteristics. It has anti-cancer properties. It can inhibit nuclear export and Rev translocation, a gene product in HIV-1 genome, at nanomolar concentrations.
    Kazusamycin A is soluble in ethanol, methanol, ethyl acetate, acetone, chloroform and benzene, practically insoluble in water, and unstable in DMSO.
    
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    Background
    
    Members of the Leptomycin complex can induce abnormal condensation of nuclei and arrest cell cycle of yeast and mammalian cells in both G1 and G2 phases, but the exact mechanism of action of Kazusmycin A is still unknown.
- Properties
  - CAS Number
    
    92090-94-3
    
    Molecular Formula
    
    C33H48O7
    
    Molecular Weight
    
    556.7
    
    Appearance
    
    Colorless film
    
    Solubility
    
    Soluble in ethanol, methanol, ethyl acetate, acetone, chloroform and benzene. Practically insoluble in water.
    
    Other Properties
    
    Source: Streptomyces spp.
    Purity Level: >95% by HPLC
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Kazusamycin A has activity against fungi including Shizosaccharomyces pombe and Rhizopus javanicus. It does not have activity against Gram-positive or Gram-negative bacteria.
    
    Microbiology Applications: Kazusamycin A and B, along with Leptomycins A and B were isolated from Streptomyces sp. strain A87-18203. These compounds were identified via a screening assay for low MW inhibitors of Rev nuclear transport. They were all found to inhibit the translocation of the regulatory protein Rev at nanomolar concentrations. Rev is need for viral replication of HIV-1 (Wolff et al, 1997).
    Kazusamycin A is the first low low MW inhibitor of nuclear export to be identified. It can be used as a tool to dissect nuclear export pathways.
    
    Eukaryotic Cell Culture Applications: Kazusamycin A has strong cytotoxic activity against HeLa cells in vitro, and 3.3 ng/ml conc. prevented cell growth (Umezawa et al, 1984).
    
    Cancer Applications: Kazusamycin A has in vitro cytotoxic activity against various human and mouse tumor cell lines. It shows in vivo activity against experimental murine tumors. It has potent in vitro and in vivo activity against P388 and L1210 cancer cell lines.
    In a study of urological cancer, Kazusamycin A was evaluated in vitro with transitional cancer cells (KU-1, T-24) and was found to induce G2 cell cycle arrest and delay the M phase of the cell cycle (Sawamura et al, 1992).

Datasheet

Kazusamycin A

Kazusamycin A

Background

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