Jacalin Coated - 96 well plates - 8 Well Strips on 12 x 8 frame

Jacalin Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.

Jacalin Plates, available in various formats, are designed for their specific affinity to molecules containing non-reducing α-D-galactosyl groups. These plates are ideal for binding carbohydrate-binding proteins and glycoproteins, particularly those found in IgA1 and cell membranes. Jacalin, a lectin derived from the seeds of Artocarpus integrifolia (jackfruit), is widely used for isolating glyco-conjugates and glycoproteins with distinct carbohydrate structures.

The Jacalin Coated 96 Well Plates are ideal for a variety of applications, including the specific binding of human IgA1 with steric orientation, the purification of human immunoglobulins (particularly IgA1), and the separation of antigen-antibody immunocomplexes. Additionally, they are used for isolating IgA1 from contaminants and stimulating T-cells. These features make the plates a versatile tool for both immunological and biochemical assays.

Jacalin Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Jacalin Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Jacalin Coated Surface is specifically designed for assays that bind carbohydrate-binding proteins and glycoproteins. These plates are highly effective for applications such as the sterically oriented binding of human IgA1, purification of human immunoglobulins, separation of antigen-antibody immunocomplexes, and the isolation of IgA1 from contaminants. Additionally, they are useful for the stimulation of T-cells, making them versatile tools for a variety of immunological and biochemical assays.

Key features of Jacalin Plates
· Enables specific binding of human IgA1 with precise steric orientation, ensuring accuracy in assays.
· Facilitates efficient purification of human immunoglobulins, particularly IgA1, improving yield and purity.
· Allows for effective separation of antigen-antibody immunocomplexes, enhancing assay sensitivity.
· Provides reliable isolation of IgA1 from contaminants, ensuring sample integrity.
· Supports T-cell stimulation, making it suitable for immunological research and applications.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Jacalin Coated Surface can be used in assays for binding carbohydrate-binding proteins and glycoproteins.
Jacalin, belonging to the lectins family, is a tetrameric (M.W. 40 kDa) carbohydrate-binding protein isolated from the seeds of jack fruit Artocarpus integrifolia agglutinin. It is well known that lectins have been used extensively for the isolation of glycol-conjugates and glycoproteins with specific carbohydrate structures.

Jacalin shows specific affinity for molecules containing non reducing α-D-galactosyl groups, usually present in the biochemical structure of IgA1 and cell membranes.

· Examples of applications for Jacalin Coated surface
Human IgA1 specific binding, sterically oriented
Purification of human immunoglobulins (especially IgA1)
Separation of immunocomplexes antigen-antibody
Separation of IgA1 from contaminants
Stimulation of T-cells

· Jacalin Coated Surfaces main features
Biomat Jacalin Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Jacalin Coated Products ensures:

Uniformity
The Jacalin Coated Products show a CV% less than 5 used as a catcher of biotinylated human IgA in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The Jacalin Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Jacalin Coated Microplates Specifications
Coating
Jacalin (M.W. 40 kDa) is coated using 100 μl/well.

Binding capacity
Microplate was saturated with biotinylated human IgA at a concentration of 6 μg/ml (600 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.

Sensitivity
Biotinylated human IgA was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.