Name: IntactProtein™ Cell-Tissue Lysis Kit
Price: 184.00 USD

- Overview
  - Key Features
    • All-in-one formula: no protease/other enzyme inhibitors or sonication required
    • Ready-to-use protocol: simply mix Reagents A & B; extraction takes as little as 15 min
    • Ultimate solution for large proteins: near-complete extraction of large proteins; no fragmentation due to no sonication
    • Assurance and peace of mind: no loss of protein PTMs such as phosphorylation, glycosylation, ubiquitination, methylation, and acetylation
    • All-around performance: suitable for mammalian cells and tissues
    
    Kit Components
    • Reagent A (40, 100, 200 μL for #415 S, M and L)
    • Reagent B (20, 50, 100 mL for #415 S, M and L)
    
    Please contact us at for specific academic pricing.
    
    Background
    
    One of the key factors influencing the result of Western blotting is the quality of proteins extracted from cells and tissues. In practice, detergent-based buffers such as radioimmunoprecipitation assay (RIPA) buffer, physical disruption such as sonication, or a combination of both, have become the standard for the extraction of proteins from cell membrane, cytoplasm, organelles, and nuclei. Although RIPA buffer (with 0.1% SDS) or its substitute like NP-40 buffer (without SDS), has been widely used to lyse mammalian cells and tissues, RIPA buffer is not as effective in extracting large proteins as it is in medium and small proteins. To increase the harvest of large proteins, most laboratories combine RIPA buffer with sonication which can physically break down DNA to reduce the viscosity of the lysates. However,sonication has the potential to break down large proteins. Furthermore, to inhibit endogenous enzyme activities, inhibitors need to be added to the RIPA buffer. For example, to reduce protein degradation, protease inhibitors such as aprotinin, leupeptin, pepstatin, and PMSF need to be added to RIPA buffer immediately before use. Similarly, sodiumfluoride and sodium orthovanadate must be added to inhibit phosphatase activities.
    
    Our IntactProtein™ Cell-Tissue Lysis Kit is formulated to solve these issues. It saves you time by avoiding adding protease, phosphatase, and other enzyme inhibitors; it can also preserve the post-translational modifications (PTMs) of the cellular proteins. Overall, this product is suitable for extracting proteins of all sizes from mammalian cells and tissues.
- Properties
  - Storage
    
    Store Reagent A at -20°C for one year.
    Store Reagent B at 4°C for one year.
    
    Note
    
    • Reagent B can be stored at room temperature for up to 3 months. For long-term storage, keep the reagent at 4 °C.
    • Precipitation may occur when Reagent B is stored at 4 °C over a prolonged period of time.
    • Precipitation does not affect product quality.
    • Precipitation will redissolve at room temperature; after the precipitation disappears, gently mix the solution.
    
    * For research use only
- Applications
  - Application
    
    Denaturing protein extraction; Western blotting (WB)
- Reference
  - He Y, B'nai Taub A, Yu L, Yao Y, Zhang R, Zahr T, Aaron N, LeSauter J, Fan L, Liu L, Tazebay R, Que J, Pajvani U, Wang L, Silver R, Qiang L. PPARγ Acetylation Orchestrates Adipose Plasticity and Metabolic Rhythms. Adv Sci (Weinh). 2023 Jan;10(2):e2204190. doi: 10.1002/advs.202204190. Epub 2022 Nov 17. PMID: 36394167; PMCID: PMC9839851. (IF = 16.3)
    Kirkpatrick LT, Daughtry MR, El-Kadi S, Shi TH, Gerrard DE. O-GlcNAcylation is a gatekeeper of porcine myogenesis. J Anim Sci. 2022 Nov 1;100(11):skac326. doi: 10.1093/jas/skac326. PMID: 36219104; PMCID: PMC9683508. (IF = 3.2)
    Yao Y, Ren Z, Yang R, Mei Y, Dai Y, Cheng Q, Xu C, Xu X, Wang S, Kim KM, Noh JH, Zhu J, Zhao N, Liu YU, Mao G, Sima J. Salidroside reduces neuropathology in Alzheimer's disease models by targeting NRF2/SIRT3 pathway. Cell Biosci. 2022 Nov 4;12(1):180. doi: 10.1186/s13578-022-00918-z. PMID: 36333711; PMCID: PMC9636768. (IF = 7.0)
    He Y, Zhang R, Yu L, Zahr T, Li X, Kim TW, Qiang L. PPARγ Acetylation in Adipocytes Exacerbates BAT Whitening and Worsens Age-Associated Metabolic Dysfunction. Cells. 2023 May 18;12(10):1424. doi: 10.3390/cells12101424. PMID: 37408258; PMCID: PMC10217233. (IF = 6.0)
    Zahr T, Boda VK, Ge J, Yu L, Wu Z, Que J, Li W, Qiang L. Small molecule conjugates with selective estrogen receptor β agonism promote anti-aging benefits in metabolism and skin recovery. Acta Pharm Sin B. 2024 May;14(5):2137-2152. doi: 10.1016/j.apsb.2024.01.014. Epub 2024 Jan 29. PMID: 38799642; PMCID: PMC11119546. (IF = 14.1)
    Yu L, Wan Q, Liu Q, Fan Y, Zhou Q, Skowronski AA, Wang S, Shao Z, Liao CY, Ding L, Kennedy BK, Zha S, Que J, LeDuc CA, Sun L, Wang L, Qiang L. IgG is an aging factor that drives adipose tissue fibrosis and metabolic decline. Cell Metab. 2024 Apr 2;36(4):793-807.e5. doi: 10.1016/j.cmet.2024.01.015. Epub 2024 Feb 19. PMID: 38378001; PMCID: PMC11070064.(IF = 27.7)
    Rimmer LA, Geisbrecht ER, Chao MD, O'Quinn TG, Woodworth JC, Zumbaugh MD. Skeletal Muscle Metabolism Is Dynamic during Porcine Postnatal Growth. Metabolites. 2024 Jun 26;14(7):357. doi: 10.3390/metabo14070357. PMID: 39057680; PMCID: PMC11279009. (IF = 4.0)
    Xu C, Mei Y, Yang R, Luo Q, Zhang J, Kou X, Hu J, Wang Y, Li Y, Chen R, Zhang Z, Yao Y, Sima J. Edaravone Dexborneol mitigates pathology in animal and cell culture models of Alzheimer's disease by inhibiting neuroinflammation and neuronal necroptosis. Cell Biosci. 2024 Apr 27;14(1):55. doi: 10.1186/s13578-024-01230-8. PMID: 38678262; PMCID: PMC11056062.. (IF = 7.0)
    Lu B, Chen S, Guan X, Chen X, Du Y, Yuan J, Wang J, Wu Q, Zhou L, Huang X, Zhao Y. Lactate accumulation induces H4K12la to activate super-enhancer-driven RAD23A expression and promote niraparib resistance in ovarian cancer. Mol Cancer. 2025 Mar 19;24(1):83. doi: 10.1186/s12943-025-02295-w. PMID: 40102876; PMCID: PMC11921584. (IF = 31.3)

Figure 4. Western blot analysis of cell lysates prepared using lysis buffers from different vendors. Protease and phosphatase inhibitor cocktail was added to Suppliers A, B, and C's lysis buffers.

Figure 5. WB analysis of tissue lysates prepared using lysis buffers from different vendors. Protease/phosphatase inhibitor cocktail was added to Suppliers A, B, and C's lysis buffers.

IntactProtein™ Cell-Tissue Lysis Kit

IntactProtein™ Cell-Tissue Lysis Kit

Background

Figure 4. Western blot analysis of cell lysates prepared using lysis buffers from different vendors. Protease and phosphatase inhibitor cocktail was added to Suppliers A, B, and C's lysis buffers.

Figure 5. WB analysis of tissue lysates prepared using lysis buffers from different vendors. Protease/phosphatase inhibitor cocktail was added to Suppliers A, B, and C's lysis buffers.

IntactProtein™ Cell-Tissue Lysis Kit

IntactProtein™ Cell-Tissue Lysis Kit

Background

We Also Recommend

BcMag™ Rapid IgG/Protein L Purification Kit

Enhanced ECL Chemiluminescent Substrate Kit

Precast Protein Plus Gel, 12%, 15 wells, Hepes-Tris