In Vitro Mammalian Cell Gene Mutation Test , V79

In Vitro Mammalian Cell Gene Mutation Test (with V79)can infer the mutagenicity of a test substance based on the following principle:Under normal conditions, cells produce hypoxanthine-guanine phosphoribosyltransferase (HGPRT). In a selective medium containing 6-thioguanine (6-TG), HGPRT catalyzes the production of nucleoside-5'-monophosphate (NMP), which gets incorporated into DNA, leading to cell death.In the presence of carcinogenic and/or mutagenic substances, mutations may occur in the structural genes on the X chromosome controlling HGPRT, resulting in the inability to produce HGPRT. Consequently, these mutated cells become resistant to 6-TG and can survive and grow in the selective medium containing 6-TG.Under conditions with or without a metabolic activation system, cell cultures are exposed to the test substance for an appropriate duration. The cells are then passaged and cultured in a selective medium containing 6-TG. Mutant cells will continue to divide and form colonies in this medium. By counting the number of mutant colonies formed and calculating the mutation frequency, the mutagenicity of the test substance can be inferred.

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