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Overview
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In Vitro Mammalian Cell Gene Mutation Test (with L5178Y) uses mouse lymphoma cells L5178Y TK+/- as the test system. Under conditions with or without a metabolic activation system, L5178Y cells are exposed to the test substance for an appropriate duration. The cells are then passaged and cultured in a selective medium containing trifluorothymidine (TFT). By counting the number of mutant colonies formed and calculating the mutation frequency, the mutagenicity of the test substance can be inferred.
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Overview