Name: ICG-Sulfo-OSu
Price: Inquiry

- Overview
  - ICG is one of the dyes that is used for determining cardiac output, hepatic function, and liver blood flow, as well as for ophthalmic angiography. It has a long excitation wavelength and emission wavelength of about 780 nm and 800 nm, respectively. Because of its long wavelength near the infrared region and low cytotoxicity, ICG is used to label antibodies for in vivo assay. However, fluorescent intensity after conjugation with protein is quite low because of the formation of H-dimer or an energy transfer to antibody molecules after excitation. Dr. Kobayashi and others reported that the use of SDS and betamecraptoethanol with the conjugate increases fluorescent intensity dramatically by diminishing hydrophobic p-p interactions and separation of IgG chains. They applied a treated ICG-conjugated daclizumab (humanized monoclonal antibody) and humanized anti-HER IgG2 monoclonal antibody for in vivo assay to specifically visualized tumors.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Fluorophore labeling
    
    Molecular Formula
    
    C49H52N3O10S2
    
    Molecular Weight
    
    930.07
    
    Appearance
    
    dark green powder
    
    Purity
    
    ≥80% (HPLC)
    
    Storage
    
    -20°C
    
    Shipping
    
    with blue ice
    
    * For research use only
- Applications
  - Application Description
    
    Labeling procedure for IgG
    1. Prepare 6.8 nmol antibody solution with pH 8.5 carbonate buffer or bicine buffer (Good’s buffer).
    2. Add 6.8-68 nmol of ICG-Sulfo-OSu/DMSO solution to the antibody solution and incubate at room temperature for 30 minutes.
    3. Purify the reaction mixture with a sephadex G50 column.

MSDS

ICG-Sulfo-OSu

ICG-Sulfo-OSu

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