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Overview
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Hydrazide Gel Kit contains all the components required to make, use, and store Hyrazide Affinity gel columns used in affinity purification procedures.Hydrazide Gel is a high efficiency glycoprotein binding medium, which will bind oxidised sugar molecules in the glycosyl groups of Anti-bodies and does not bind to amino acids .This important function makes it specific to immune-globulins where the glycosylated area is distinctly located away from the hyper-variable binding region. By using Hydrazide gel to exploit this antibodies may be linked to a solid phase support via the oxidised glycosyl groups. Thus preserving the antibody integrity, which no other linking method can guarantee.The method employs the oxidation of sugar molecules with sodium meta-periodate which converts vicinal pairs of hydroxyl groups to aldehydes, (-CHO),it is these which bind hydrazide agarose.
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Overview