Human IFN-γ T Cell ELISPOT

Human IFN-γ T Cell ELISPOT

Catalog Number:
EK02494159UCY
Mfr. No.:
CT230-PR2/PR5
Price:
$754
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      • Overview
        • The T cell ELISPOT assay is a simple and sensitive immunoassay for the detection of protein secreting T cells at the single cell level.

          Staining: Enzymatic
          Characteristics: PVDF

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          • Coating antibodies
          • Biotinylated detection antibodies
          • Streptavidin-Horseradish Peroxidase
          • AEC coloring system
          • Blocking stock solution
          • Dilution buffer R
          • Tween-20
          • Detailed manual

          * For research use only.

      • Applications
        • Application Description
          The ELISPOT assay is one of the most sensitive assays to monitor ex vivo cellular immune responses at the single cell level. The assay can accurately detect secreted proteins, such as cytokines, released by T cells in response to an antigen. The cell suspensions used in the test can be from blood (PBMC), lymphoid, spleen, bone marrow or CNS tissue.
      • Reference
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          Bererhi, Lynda, et al. Transpl. Int. 2012 May;25(5): 545-54
          • Radiotherapy and High-Dose Interleukin-2: Clinical and Immunological Results of a Proof of Principle Study in Metastatic Melanoma and Renal Cell Carcinoma.
          Bulgarelli, Jenny, et al. Front Immunol 2021;12: 778459
          • Humoral and cellular immune responses after influenza vaccination in kidney transplant recipients.
          Candon, S, et al. Am. J. Transplant. 2009 Oct;9(10): 2346-54
          • B7-H1 up-regulation on myeloid dendritic cells significantly suppresses T cell immune function in patients with chronic hepatitis B.
          Chen, Liangen, et al. J. Immunol. 2007 May 15;178: 6634-41
          • Dynamic changes of cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and natural killer T (NKT) cells in patients with acute hepatitis B infection.
          Li, Jun, et al. Virol. J. 2011 May 02;8: 199
          • Novel immunodominant peptide presentation strategy: a featured HLA-A*2402-restricted cytotoxic T-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein.
          Liu, Jun, et al. J. Virol. 2010 Nov;84: 11849-57
          • HLA-class II restricted TCR targeting human papillomavirus type 18 E7 induces solid tumor remission in mice.
          Long, Jianting, et al. Nat Commun 2024 Mar 13;15(1): 2271
          • Methyldopa blocks MHC class II binding to disease-specific antigens in autoimmune diabetes.
          Ostrov, David A, et al. J. Clin. Invest. 2018 May 01;128: 1888-1902
          • Stability Program in Dendritic Cell Vaccines: A "Real-World" Experience in the Immuno-Gene Therapy Factory of Romagna Cancer Center.
          Pancisi, Elena, et al. Vaccines 2022 Jun 23;10(7): 999
          • Identification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8.
          Tang, Baishan, et al. Mol Med Rep 2015 Aug;12: 1741-52
          • A unique immune signature in blood separates therapy-refractory from therapy-responsive acute graft-versus-host disease.
          van Halteren, Astrid G S, et al. Blood 2023 Mar 16;141(11): 1277-1292
          • Identification of Mutated Peptides in Bladder Cancer From Exomic Sequencing Data Reveals Negative Correlation Between Mutation-Specific Immunoreactivity and Inflammation.
          Wang, Chen, et al. Front Immunol 2020;11: 576603
          • Comparable antigen-specific T cell responses in vaccinees with diverse humoral immune responses after primary and booster BBIBP-CorV vaccination.
          Wei, Dong, et al. Emerg Microbes Infect 2022 Sep 27;11(1): 2474-2484
          • Influence on the immune function of the human peripheral blood mononuclear cells transfected by retrovirus-mediated HSV-tk gene.
          Xue, Xing-kui, et al. Chin. Med. J. 2006 Jun 20;119: 1038-41
          • Age-Related Immunoreactivity Profiles to Diverse Mycobacterial Antigens in BCG-Vaccinated Chinese Population.
          Yang, Qing-Yuan, et al. Front Immunol 2020;11: 608220
          • Fulminant type 1 diabetes mellitus exhibits distinct clinical and autoimmunity features from classical type 1 diabetes mellitus in Chinese.
          Zheng, Chao, et al. Diabetes Metab. Res. Rev. 2011 Jan;27: 70-8
          • A pilot study of multi-antigen stimulated cell therapy-I plus camrelizumab and apatinib in patients with advanced bone and soft-tissue sarcomas.
          Zhou, Yan, et al. BMC Med 2023 Nov 29;21(1): 470

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