Human FOXP3 TSDR Pyrosequencing methylation assay

Regulatory T-cells (Tregs) prevent autoimmune disease by inhibiting self-reactive T-cells. FOXP3 (forkhead box P3) is a transcription factor that is found in Tregs. It controls the expression of genes involved in regulating the immune system, making FOXP3 the master regulator that controls the development and function of Tregs. The methylation status of the FOXP3 gene can be used to measure the proportion of Tregs in a larger population of leukocytes. Our FOXP3 panel is a set of validated DNA methylation assays studying the methylation status of the FOXP3 gene and the regulation of genes (such as CTLA4 and CD25) important to Treg function.

Gene Name: Human FOXP3, Intron 1 TSDR Region
FOXP3 (forkhead box P3), Location: -2330 to -2263 from ATG 9 0
9 of CpGs

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Background

Forkhead-box protein P3 (Foxp3) is a major transcription factor that is necessary for induction of the immunosuppressive function of regulatory T cells (Tregs). It is considered to be the most specific marker for Tregs in tumors, with its expression leading to poor survival rates in some types of cancer and improved survival rates in other types. Studies have shown that epigenetic modifications contribute to the stable expression of FOXP3 and other molecules associated with Treg cell function. These modifications include demethylation of CpG sites in the conserved non-coding sequence 2 (CNS2), Treg specific demethylated region (TSDR), proximal promoter, and upstream enhancer regions of the gene. Understanding the epigenetic regulation of FOXP3 and associated genes is critical in developing new approaches to autoimmune disease and cancer treatment.